WebJun 14, 2024 · implemented new guppy version and sup model dna_r9.4.1_450bps_sup.cfg" implemented support for rapid barcoding kit via --rapid; … WebJul 1, 2024 · In the command above we. select hits from a bam file mapping to a specific region; extract the reads as fastq; compress the fasta file with pigz; write the fasta hits to …
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WebTo convert a FAST5 dataset to a single BLOW5 file and then convert back to FAST5. slow5tools f2s fast5_dir -d blow5_dir -p 8 #Merge all the slow5 files in to a single file … Sludge biomass was sampled from the anaerobic digester at Fredericia Wastewater Treatment Plant in Denmark (latitude 55.552219, longitude 9.722003) at multiple time points and stored as frozen 2 mL aliquots at −20 °C. For the Zymo sample, the ZymoBIOMICS HMW DNA Standard D6322 (Zymo Research) … See more DNA was extracted from the anaerobic digester sludge using the DNeasy PowerSoil Kit (Qiagen) following the manufacturer’s … See more Library preparation was carried out using the ligation sequencing kits (Oxford Nanopore Technologies) SQK-LSK109 and SQK-LSK112 for sequencing on R.9.4.1 and the R.10.4 flowcells, respectively. … See more DNA concentrations were determined using the Qubit dsDNA HS kit and were measured with a Qubit 3.0 fluorimeter (Thermo Fisher). … See more The anaerobic digester Illumina libraries were prepared using the Nextera DNA library preparation kit (Illumina), while the Zymo Mock sample was prepared with the NEB Next Ultra II … See more lightest bodied white wine
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