Flottilin antibody

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August undefined, 2024

WebFeb 24, 2024 · Datasheets. Flotillin-1 Antibody (C-7) is a mouse monoclonal IgG 1 κ, cited in 17 publications, provided at 200 µg/ml. raised against amino acids 324-427 of Flotillin-1 of human origin. Anti-Flotillin-1 Antibody (C-7) is recommended for detection of Flotillin-1 of mouse, rat and human origin by WB, IP, IF and ELISA. WebWestern blot was performed using Anti- Flotillin 1 Polyclonal Antibody ( Product # PA5-19713) and a 47kDa band corresponding to Flotillin 1 …

Anti Flotillin-1 Antibody (PrecisionAb Polyclonal Antibody) - Bio …

WebAnti-Flotillin 1 antibodies are available from several suppliers. In humans, this protein is encoded by the gene FLOT1. The protein may also be known as integral membrane component of caveolae. The expected protein mass is 47.4 kDa, but there are 2 reported isoforms. Variants of this antigen may also be found in other species, including plant ... WebDescription. Flotillin 1 antibody LS-B9180 is an unconjugated goat polyclonal antibody to Flotillin 1 (FLOT1) (aa415-427) from human. It is reactive with human, bovine, dog and other species. Validated for IHC, Peptide-ELISA … shop for refrigerators by dimensions

Recombinant Anti-Flotillin 1 antibody [EPR6041] - BSA and Azide …

WebThe blot was probed with Flotillin 2 Polyclonal Antibody (Product # PA5-30033, 1:500 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 … WebRed blood cell antibody identification: Single antibody specificity; Multiple antibody specificities; Antibody to high prevalence antigen; Antibody to low prevalence antigen; … WebFlotillin 1 Antibody (PA5-17127) in WB. Western blot was performed using anti-Flotillin 1 Polyclonal Antibody (Product # PA5-17127) and a 47kDa band corresponding to Flotillin 1 was observed across all the cell lines and tissue lysates tested except THP1 which is reported negative for Flotillin 1 expression. Whole cell extracts (30 µg lysate ... shop for rent bakersfield ca

Flotillin 1 Antibodies, ELISA Kits - antibodies-online.com

Flotillin 1 Polyclonal Antibody (PA5-17127) - Thermo Fisher Scientific

WebAntibody dilution 1:1000. Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug rat brain lysate/extract. Antibody dilution 1:1000. Western Blot: Flotillin 2 Antibody [NBP1-30881] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody ... WebBiochem/physiol Actions. Flotillin-2 is a protein encoded by the FLOT2 gene in humans. Flotillins are involved in cell adhesion processes and play a role during breast cancer progression. FLOT2 especially plays a functional role in cell-cell adhesion in non-malignant epithelial cells. 6] It may have an essential function in intestinal ... shop for postersWebidentity and significance of flotillin cross-reacting determinant remains unknown. Here, we examine the cell type and tissue-specific expression of the flotillins using a novel monoclonal antibody probe that recognizes only flotillin-1. A survey of cell and tissue types demonstrates that flotillins 1 and 2 have a complementary tissue distribution. shop for rent albury

"WebJul 3, 2024 · Blots were probed with primary antibodies against positive (caveolin and flotillin) and negative (clathrin and Na + /K + ATPase) lipid raft markers in addition to NTCP and OCT1 overnight at 4 °C in the respective blocking solution. Blots were washed three times with TBS-T before incubation with HRP-conjugated secondary antibodies in 2.5% … " - Flottilin antibody

Flottilin antibody

PE Anti-Flotillin 1 antibody [EPR6041] (ab225165) Abcam

WebMonoclonal Antibody Infusion for COVID-19. Based on the National Institutes of Health guidance, Piedmont Healthcare has discontinued offering monoclonal antibodies …

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WebIn addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found in compartments of the endocytic and … WebOct 4, 2024 · Monoclonal antibodies are designed in a lab to attack a specific part of a virus. Dr. M.G. Finn, of Georgia Tech , points out that laboratory created antibodies …

WebWestern blot of anti-Flotillin-1 antibody (clone W16108A) and isotype-matched IgG2a control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Flotillin-1 antibody, or rat IgG2a, κ isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. WebFlotillin-1 Antibody (A303-422A) in WB. Detection of human and mouse Flotillin-1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from …

WebFlotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are … WebDec 11, 2005 · Antibodies against flotillin-1, Shp2 (a cytosolic protein) and ERp72 (an endoplasmic reticulum membrane protein) were used separately; specificity of the flotillin-1 antibody was confirmed in ...

WebImmunofluorescence staining of Jurkat cells with purified ab133497 at a working dilution of 1/500, counter-stained with DAPI. The secondary …

WebMonoclonal Antibody for studying flotillin-1. Cited in 71 publications. Validated for WB, IP, IHC, IF. Available in 2 sizes. Highly specific and … shop for reebok indiaWebAnti-Flotillin 1-CY3 ™ antibody is suitable for direct immunofluorescence at a concentration of 2-5μg/mL using human HEK-293T or HeLa cells. Biochem/physiol Actions. Flotillin 1 is directly connected to the plasma membrane. The association of flotillin 1 depends on the palmitoylation in a conserved Cys34 residue, whereas the association of ... shop for prom dressWebMar 8, 2024 · ab247493 is the carrier-free version of ab78178. Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency. This conjugation-ready format is designed for use with fluorochromes, metal ... shop for promethean prm-35 projectorWebHuman Flotillin-1 recombinant protein corresponding to amino acids 1-270 expressed in E. coli. Formulation. This antibody is provided in 50% glycerol in aqueous buffered … shop for rent boiseWebAll lanes : Anti-Flotillin 1 antibody (ab41927) at 1 µg/ml Lane 1 : Lung (Human) Tissue Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : … shop for rent bath high streetWebAll lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/1000 dilution Lane 1 : Wild-type HEK293T cell lysate Lane 2 : FLOT1 knockout HEK293T cell lysate Lane 3 : A431 cell lysate Lane 4 : HAP1 cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/10000 dilution Predicted … shop for rent calgaryWebFlotillin-1, encloded by the FLOT1 gene, is a caveolae-associated, integral membrane protein. The function of flotillin-1 has not been determined (provided by RefSeq, Jul 2008). Goat anti Human flotillin-1 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. shop for rent cheyenne wy